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姓　　名： 王皓毅
学　　科： 发育生物学
电话/传真： +86-10-82619213 / 
电子邮件： wanghaoyi@ioz.ac.cn
通讯地址： 北京市朝阳区大屯路甲3号 中国科学院动物研究所 北京干细胞与再生医学研究院 干细胞与生殖生物学国家重点实验室 100101
更多信息： 基因工程技术研究组     

简历介绍：

　　王皓毅，博士，研究员，中国科学院动物研究所干细胞与生殖生物学国家重点实验室副主任，基因工程技术研究组组长。2009年于美国华盛顿大学获得分子细胞生物学博士学位，开发了基于转座子的Calling Card技术。2009年至2014年在麻省理工学院Whitehead研究所Rudolf Jaenisch实验室进行博士后研究，从事全能性干细胞和小鼠中基因编辑技术的开发和应用。2014年加入中国科学院动物研究所，担任基因工程技术研究组组长。在近年研究中取得多项成果，包括在人类多能性干细胞和CAR-T细胞中建立了高效的基因编辑方法；建立基因编辑以及胚胎电转构建小鼠模型方法；建立人类naive胚胎干细胞培养条件，并基于此实现人类X染色体随机失活的模拟；建立原位基因表达调控技术CRISPR-on和Casillio系统等。相关研究成果发表在Cell、Nature Biotechnology、Cell Stem Cell、Genome Research、Cell Research等杂志上，已发表SCI学术论文五十余篇，其中（共同）第一作者和通讯作者文章37篇，总引用次数已过万次 (google scholar统计)。回国后已获得国家自然科学基金优秀青年基金、面上项目、中科院干细胞先导专项以及科技部干细胞与再生医学重点研发计划等经费资助。

研究领域：

　　基因工程技术的开发和应用

　　近期研究方向：

　　1. 开发源头创新的基因工程技术。

　　2. 在T细胞和干细胞中建立和优化基因编辑技术，开发新型疾病治疗方法。

　　3. 利用人类胚胎干细胞技术研究早期胚胎发育中的X染色体失活现象的分子机制。

社会任职：

获奖及荣誉：

承担科研项目情况：

代表论著：

（*共同第一作者, #通讯作者）

  1. Li N*, Tang N, Cheng C, Hu T, Wei X, Han W, Wang H#. “Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway.” OncoImmunology, 2020 Sep 24 Online.
  2. Sun W*, Wang H#. (2020). “Recent advances of genome editing and related technologies in China”. Gene Therapy, 2020 Aug; 27(7-8): 312-320. 
  3. An C*, Feng G*, Zhang J, Cao S, Wang Y, Wang N, Lu F, Zhou Q, Wang H#. “Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation.” Cell Stem Cell, 2020 Sep; 27（3）: 482-497.e4. 
  4. Tang N*, Cheng C*, Zhang X, Qiao M, Li N, Mu W, Wei X, Han W, Wang H#. “TGF β Inhibition via CRISPR Promotes the Long-Term Efficacy of CAR-T Cells Against Solid Tumors.” JCI Insight, 2020 Feb; 5(4): e133977. 
  5. Cao Y*, Sun W*, Wang J, Sheng G, Xiang G, Zhang T, Shi W, Li C, Wang Y, Zhao F#, Wang H#. “Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double- stranded DNA at 37 °C.” Cell Discovery, 2019; 5:38.
  6. Wang H#，Yang H#. “Gene-edited babies: What went wrong and what could go wrong.” PLOS Biology, 2019 Apr 30 Online.
  7. Mu W*, Tang N*, Cheng C*, Sun W, Wei X, Wang H#. “In vitro transcribed sgRNA causes cell death by inducing interferon release.” Protein & Cell, 2019 Jan 7 Online. 
  8. Cheng C*, Tang N*, Li J, Cao S, Zhang T, Wei X, Wang H#. “ Bacteria-free minicircle DNA system to generate integration-free CAR-T cells.” Journal of Medical Genetics, 2019 Jan; 56(1): 10-17. 
  9. Xiang G*, Ren J*, Hai T*, Fu R, Yu D, Wang J, Li W, Wang H#, Zhou Q#. “Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs.”  Cellular and Molecular Life Sciences. 2018 Dec; 75(24): 4619-4628.  
  10. Xiang G, Wang H#. “Extended pluripotent stem cells facilitate mouse model generation.”  Protein & Cell. 2018 Aug 21. doi: 1007/s13238-018-0573-0.
  11. Mu W, Zhang Y, Xue X, Liu L, Wei X, Wang H#.” 5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system.” Protein & Cell, 2018. June Online. 
  12. Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H#.“CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.” Frontiers of Medicine, 2017 Dec;11(4):554-562.
  13. Xiang G, Zhang X, An C, Cheng C, Wang H#.“Temperature effect on CRISPR-Cas9 mediated genome editing.”Journal of Genetics and Genomics, 2017 Apr;44(4):199-205.
  14. Liu X, Zhang Y, Cheng C, Cheng WA, Zhang X, Li N, Xia C, Wei X, Liu X, Wang H#. “CRISPR-Cas9 mediated multiplex gene editing in CAR-T cells.” Cell Research, 2017 Jan;27(1):154-157.
  15. Burgess S, Cheng L#, Gu F, Huang J, Huang Z, Lin S#, Li J, Li W, Qin W, Sun W, Songyang Z, Wei W#, Wu Q, Wang H#, Wang X, Xiong JW, Xi J, Yang H, Zhou B, Zhang B. “Questions about NgAgo” Protein Cell, 2016 Dec;7(12):913-915.
  16. Wang W,Kutny PM, Byers SL, Longstaff CJ, DaCosta MJ, Pang C, Zhang Y, Taft RA, Buaas FW, Wang H#. “Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increased the efficiency of model creation.” Journal of Genetics and Genomics, 2016 May 20;43(5):319-27.
  17. Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H#.“Generating Mouse Models Using CRISPR-Cas9 Mediated Genome Editing.” Current Protocols in Mouse Biology, 2016 Mar 1;6(1):39-66. 
  18. Cheng AW*#, Jillette N*, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H#. “Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.” Cell Research, 2016 Feb;26(2):254-7. 
  19. Wiles MV, Qin W, Cheng AW, Wang H#. “CRISPR-Cas9 mediated genome editing and guide RNA design.” Mammalian Genome, 2015 Oct;26(9-10):501-10.
  20. Qin W, Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen YG, Wang H#. “Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease.” Genetics, 2015 Jun;200(2):423-30.
  21. Theunissen T*, Powell B*, Wang H*, Mitalipova M, Faddah D, Reddy J, Fan Z, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty M, Young R, Gray N, Jaenisch R. “Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency.”Cell Stem Cell, 2014 Oct 2; 15(4):471-87.
  22. Yang H, Wang H, Jaenisch R. “Generating genetically modified mice using CRISPR/Cas-mediated genome engineering.” Nature protocols, 2014 Aug; 9(8):1956-68.
  23. Maetzel D*,Sarkar S*, Wang H*, Mosleh LA, Cheng AW, Xu P, Gao Q, Mitalipova M, Jaenisch R. “Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells.” Stem Cell Reports, 2014 May 15; 2(6):866-80.
  24. Cheng AW*, Wang H*, Yang H, Shi L, Katz Y, Rangarajan S, Theunissen TW, Shivalila CS, Dadon DB, Jaenisch R. “Multiplexed activation of endogenous genes by CRISPR-on, a RNA-guided transcriptional activator system.” Cell Research, 2013 October 1; 23(10): 1163-1171.
  25. Yang H*, Wang H*, Shivalila CS*, Cheng AW, Shi L, Jaenisch R. “One-step generation of mice carrying reporter and conditional allele by CRISPR/Cas mediated genome editing” Cell, 2013 September 12; 154(6):1370-1379.
  26. Faddah D, Wang H, Buganim Y, Cheng AW, Jaenisch R. “Expression of Nanog is biallelic and equally variable as other pluripotency factors.” Cell Stem Cell, 2013 Jul 3;13(1):23-9.
  27. Wang H*, Hu YC*, Markoulaki S, Welstead GG, Shivalila CS, Cheng AW, Pyntikova T, Dadon D, Voytas DF, Bogdanove AJ, Page DC, Jaenisch R. “TALEN-mediated editing of the Mouse Y Chromosome.” Nature Biotechnology, 2013 Jun; 31(6):530-2.
  28. Wang H*, Yang H*, Shivalila CS*, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. “One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas mediated genome engineering.” Cell, 2013 May 9;153(4):910-8. 

写给考生的话：

　　真正好的科学研究是充满乐趣和成就感的，希望我们能一起体验科学的魅力。