Faculty Profile
 
  CAI Gang
     
Department: School of Life Sciences
Mailing Address:
No. 443 Huangshan Rd, Hefei, Anhui, PRC 230027
Postal Code:
230037
Phone:
+86-551-63603802
Fax:
86-551-63603802
Homepage:
http://cii.ustc.edu.cn/cailab/
 
       

Research Profile

We are interested in examining the structures and functional interactions of key transcriptional complexes responsible for eukaryotic transcription, aiming at a structural understanding of the mechanism of transcription and its regulation. Regulation of eukaryotic gene expression is largely focused on the transcription initiation process, which involves assembly of the gigantic pre-initiation complex (PIC). Structural analysis the PIC complex is critical to elucidate the mechanism of transcription initiation; however, it is complicated by PIC's large size (> 3M Da), complexity (~60 proteins) and highly dynamic nature. Single particle electron microscopy (EM) is a powerful technique to study both the structure and dynamics of macromolecular complexes in their close to native state. My research is directed towards understanding both the structure and regulation of the Mediator complex, which plays a critical role in promoting assembly as well conveying regulatory signals from DNA-binding transcription factors to the PIC. An important aspect will be devoted to determine the structures of larger, key transcription complexes in which Mediator interacts with the PIC components to attain a structural understanding of transcription initiation.
 
     
Selected Publications
1) 3.9 Å structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP , Science , 2017 , 2017.12
2) Architecture of the Saccharomyces cerevisiae NuA4/TIP60 complex , Nature Communications , 2018 , 2018
3) Structure of the intact ATM/Tel1 kinase , Nature Communications , 2016 , 2016
4) State-of-The-Art of Single Particle EM. , Acta Biophysica Sinica , (2010) , 26, 560-69
5) Redefining the modular organization of the core Mediator complex , Cell Research , 2014 , 2014.07
6) Architecture of SWI/SNF Chromatin Remodeling Complex , Protein & Cell , 2018 , 2018
7) Structural flexibility and functional interaction of Mediator Cdk8 module(SCI收录) , Protein Cell , 2013 , 4, 911-920
8) AI-2 quorum sensing negatively regulates rbf expression and biofilm formation in Staphylococcus aureus , International Journal of Medical Microbiology , 2017 , 2017.06
9) Crystal and EM structures of human phosphoribosyl pyrophosphate synthase I (PRS1) provide novel insights into the disease-associated mutations , PLos One , 2015 , 2015
10) Crystal structure of DnaT84–153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode , Nucleic Acids Res , 2014 , 2014
11) Structural basis for receptor recognition and pore formation of a zebrafish aerolysin‐like protein , EMBO Reports , 2016 , 2016
12) Structural investigation of the interaction between the tandem SH3 domains of c-Cbl-associated protein and vinculin , Journal of Structural Biology , 2014 , 2014
13) Architecture of the Mediator head module , Nature , 2011 , 475, 240-3
14) Mediator head module structure and functional interactions. (SCI 收录) , Nat Struct Mol Biol , (2010) , 17, 273-9
15) Interaction of the mediator head module with RNA polymerase II. , Structure , 2012 , 2012
16) Mediator structural conservation and implications for the regulation mechanism (SCI收录) , Structure , (2009) , 17, 559-67
17) A TFIIH-associated mediator head is a basal factor of small nuclear spliced leader RNA gene transcription in early-diverged trypanosomes (SCI收录) , Mol Cell Biol. , (2010) , 30, 5502-13
18) Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes. , Nat Struct Mol Biol , 2011 , 18, 1196-203
19) Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability. , Appl Environ Microbiol , (2004) , 70, 2764-70
20) Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans (SCI收录) , FEMS Microbiol Lett , 2005 , 249, 15-21
 
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