Faculty Profile
 
  CAI Gang
     
Department: School of Life Sciences
Mailing Address:
No. 443 Huangshan Rd, Hefei, Anhui, PRC 230027
Postal Code:
230037
Phone:
+86-551-63603802
Fax:
86-551-63603802
Homepage:
http://cii.ustc.edu.cn/cailab/
 
       

Research Profile

We are interested in examining the structures and functional interactions of key transcriptional complexes responsible for eukaryotic transcription, aiming at a structural understanding of the mechanism of transcription and its regulation. Regulation of eukaryotic gene expression is largely focused on the transcription initiation process, which involves assembly of the gigantic pre-initiation complex (PIC). Structural analysis the PIC complex is critical to elucidate the mechanism of transcription initiation; however, it is complicated by PIC's large size (> 3M Da), complexity (~60 proteins) and highly dynamic nature. Single particle electron microscopy (EM) is a powerful technique to study both the structure and dynamics of macromolecular complexes in their close to native state. My research is directed towards understanding both the structure and regulation of the Mediator complex, which plays a critical role in promoting assembly as well conveying regulatory signals from DNA-binding transcription factors to the PIC. An important aspect will be devoted to determine the structures of larger, key transcription complexes in which Mediator interacts with the PIC components to attain a structural understanding of transcription initiation.
 
     
Selected Publications
1) Architecture of the Mediator head module , Nature , 2011 , 475, 240-3
2) Mediator head module structure and functional interactions. (SCI 收录) , Nat Struct Mol Biol , (2010) , 17, 273-9
3) A TFIIH-associated mediator head is a basal factor of small nuclear spliced leader RNA gene transcription in early-diverged trypanosomes (SCI收录) , Mol Cell Biol. , (2010) , 30, 5502-13
4) State-of-The-Art of Single Particle EM. , Acta Biophysica Sinica , (2010) , 26, 560-69
5) Mediator structural conservation and implications for the regulation mechanism (SCI收录) , Structure , (2009) , 17, 559-67
6) Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans (SCI收录) , FEMS Microbiol Lett , 2005 , 249, 15-21
7) Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability. , Appl Environ Microbiol , (2004) , 70, 2764-70
8) Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes. , Nat Struct Mol Biol , 2011 , 18, 1196-203
 
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